How DNA Methylation Affects Gene Expression

The methylation cycle is important to regulating how the information from our genes is used by turning the genes “on” or “off.” In methylated DNA areas, the methylation process becomes a barrier and prevents proteins from binding to those sections and DNA and activating them. Because of this, these genes then become deactivated or “turned off.” But when our DNA is unmethylated, genes become actives or “turned on,” which then allows for the production of RNA and the blend of proteins.

DNA Methylation Patterns and Detection

The patterns of methylation are not permanent. When our genes get passed on, these patterns are mostly erased and reset from generation to generation. This ensures that there is diversity in our genes and promotes the proper development of healthy genetics.

There are a variety of techniques that can be used to detect these methylation patterns, such as methylation-specific PCR (MSP) and whole genome bisulfite sequencing (BS-Seq). Detecting these patterns can give us information about the methylation status across our DNA.

Who Might Benefit from a Methylation Test?

A genetic methylation test can provide helpful information to people with a wide range of health conditions and concerns. Those who might benefit from getting a methylation test could be those with:

• Cardiovascular disease
• Psychiatric and mood disorders like depression, anxiety, schizophrenia, bipolar disorder, and autism
• Impaired ability to detox, whether due to toxin exposure, hormone therapy, or alcohol consumption
• Neurological conditions like Parkinson’s disease
• Chronic fatigue
• Cognitive decline
• Cancer risk

What Can You Learn From a DNA Methylation Analysis?

A genetic methylation test can give you information about your cellular processes, how your DNA is regulated, the development of diseases, and if you have any genetic predispositions that might impair the methylation process. By learning about the genetic variations in your DNA methylation profiles, you can determine what changes you can make to your lifestyle and diet to improve methylation and possibly reduce your risk of chronic illness. Here are a few of the things that a methylation test can analyze:

MTHFR

The MTHFR gene provides our bodies with instructions on how to make the MTHFR enzyme that helps our bodies to change vitamin B9 (folate) into its active form, 5-MTHF. If the MTHFR gene does not work properly, we might be unable to convert folate, which would lead to a folate deficiency. A folate deficiency can impact our mood, energy, digestion, etc. This enzyme also helps our bodies absorb and process the amino acid homocysteine. This can be a problem because elevated homocysteine levels can lead to heart disease.

MTR

This gene gives our bodies instructions on making the methionine synthase (MS) enzyme, which helps the body convert homocysteine into methionine. Methylation of this gene is essential because the MS enzyme can’t convert the homocysteine without methylated B12. If you have a variant of this gene, you might not be able to metabolize homocysteine, which would increase the risk of cardiovascular disease, cognitive problems, digestive issues, and cancer.

MTRR

The MTRR gene instructs our bodies on how to make the methionine synthase reductase (MSR) enzyme, which is needed for the MS enzyme that we mentioned above to work correctly. These two enzymes (MS and MSR) work together to modify homocysteine into methionine. Because of this, genetic differences in the MTRR gene can also cause high levels of homocysteine, which can cause depression, hypertension, and Parkinson’s disease.

COMT

Like the other genes, this gene gives our bodies information on how to make another enzyme, called catechol-O-methyltransferase, or COMT for short. COMT helps our bodies absorb and process neurotransmitters like dopamine and epinephrine. COMT is especially important in the prefrontal cortex – the area of our brains responsible for emotion, planning, and self-control. Because of that, studies of variants in this gene show that it could be linked to an increased risk of behavior problems and mental health disorders.

What is a Critical Biochemical Methylation Pathway?

A “critical biochemical methylation pathway” is a series of chemical reactions that regulate various functions in the body, like producing neurotransmitters, gene expression, repairing DNA, detoxification, and other vital functions in the body. These functions all focus on keeping our cells healthy and functioning properly. This crucial biochemical process happens when methyl groups are added to various molecules in our bodies, which essentially becomes something like a biological switch, turning specific genes “on” or “off” depending on what the body needs. This process is important because any disruptions in methylation can cause a variety of health conditions.

Why Should I Get a Methylation Panel?

Getting a methylation panel can help you understand how your methylation pathway is working and help identify any possible health concerns. Getting a genetic methylation test can help you uncover differences or variations in your genes, which can be related to a variety of health concerns. Studies have shown some of the effects of these variations, such as:

• An increase in homocysteine levels – a biomarker for inflammation, which can be related to cognitive decline, heart disease, etc.
• The inability to turn vitamin B9 (folate) into its active form (methylfolate), which would result in a deficiency of this important vitamin and its benefits.
• Variations in genes that may be related to cognitive and mood disorders.
• Changes in the methylation pathway that could disrupt essential processes that balance hormone metabolism and the ability to detox from harmful substances. 

Understanding your methylation genes can give you helpful information on how genetic and environmental factors can impact your health and what lifestyle habits you might want to change. For example, if your results show that you have an MTHFR variation, you will know to increase your intake of folate-rich foods like broccoli, liver, leafy greens, etc., to support better brain and heart health.

How to Test for Methylation

Genetic testing for methylation is done by analyzing markers in your DNA that determine methylation levels, which can give you information about any possible genetic predispositions or health risks. Testing for methylation is usually done with blood or saliva samples. The samples are then collected and sent to a lab to be tested and analyzed for specific patterns in your DNA.

Is DNA Testing Worth it?

Deciding whether to test for DNA depends on your health and genes. If you want to know more about potential genetic predispositions or if members of your family have a history of medical conditions, DNA testing could be beneficial. Knowing your genetic makeup can give you valuable information on how to make informed decisions about your health and lifestyle.

Some of the major benefits of DNA testing are:

• Being able to identify genetic predispositions to certain diseases.
• The ability to create a personalized diet and nutrition plan that matches your genetic needs.
• Optimizing your fitness routines based on genetic tendencies.
• The ability to assess potential side effects of medications and certain foods.
• Being able to improve overall preventive healthcare strategies.

Conclusion

For those who might be concerned about developing genetic health conditions and diseases, a genetic methylation test can be a good start to understanding how gene expression and methylation patterns might be related to these concerns. DNA methylation tests can help us identify areas where our DNA might be lacking and how to manage these areas to improve our health.